Hypoglycemic effect of isonicotinic acid hydrazide in normal, alloxanized and pancreatectomized dogs

Abstract

The action of the isonicotinic acid hydrazide on the gly­ cemia of normal, treated with aloxan and pancreatectomized dogs is studied, using the same method as on our previous works.

The results are expressed on graphics 1, 2 and 3, cor­ responding the first to normal dogs using doses of 50, 15 and 10 milligrams of hydrazide per Kg. orally. The second cor­ responds to normal and pancreatectomized dogs at doses of 15 milligrams per Kg. rectally and the third to dogs treated with aloxan at doses of 1.5 milligrams per Kg. orally, as onthe first graphic.

The results obtained with normal dogs are opposed in the glycemia at 4 hours in that with a dose of 50 milligrams we sce an increase of 9 % which with a dose of 15 milligrams corresponds to a glycemic decrease of 24 % and on the con- trarv on the eigth hour the greatest decrease corresponds to a dose of 50 milligrams which reaches a valué of 40 % against a dose of 10 milligrams which only reaches a decrease of 20 %.

On the graphic corresponding to the pancreatectomized dogs a glycemia increase of 25 % is observed after 4 hour and of 38 % after 6 and 8 hours. On the same graphic we inelude a curve corresponding to normal dogs using the same dose rectally.

On the third graphic, corresponding tb dogs' treated with aloxan, we observe a continuous decrease which starts with 10 % after 4 hours and reaches 22 % after 8 hours passing through 16 % after 6 hours.

From these results it isdcduced that the hypoglycemic action (and the toxic, which appears in 25 % of the dogs when it is administered at a dose of 50 milligrams per Kg of weight) of this substance which is being studied is due to the —NH—NH2 group because the —CO—NH2 rest united to the same pyridine nucleous does not provoke the effeets in- dicated before.