Applicability of the successive absorption method of studies of intestinal absorption of aminoacids

Abstract

A study has been made in vivo, using the successive absorption technique of Sois and Ponz, of the intestinal absorption of glycine in rats.

The absorption remains constant (p < 0.01) through eight successive absorption periods of 30 minutes, from Solutions of glycine of the same concentraron, within the limits of 20 to 80 mM. The constancy disappears with glycine 160 mM, and a Progressive fall is noted in the speed of the absorption throughout the successive periods, that in the eighth being 26.4 % lower than that in the first.

The velocity of glycine absorption increases with the con­ centraron of the solution to be absorbed, and is of 2.4, 4.8, 7.5 and 10.3 /xM per cm. of intestine in 30 minutes from Solu­tions of 20, 40, 80 and 160 mM respectively, presenting a clear cendency to saturation.