Studies on T cells in newborns. Higher reactivity of umbilical cord blood lymphocytes to PHA as measured by whole blood microtechnique

R. Ruiz-Requena
F. Garrido
A. Martín-Andrés
C. Osorio
53

Abstract

The capacity of human foetal lymphocytes to respond to PHA and to form E-rosettes have been compared with data from adult individuals. For this purpose a microculture system that uses whole blood and avoids the problems of lymphocyte separation, has been developed. Foetal lymphocytes reached optimal stimulation with lower dosis of PHA (31,2 microgram/ml) as compared with adult cells (125-252 microgram/ml). However their quantitative response (measured by 14C-thymidine uptake) was equal in both groups. In addition, peripheral T cells (E-rosetting cells) reached values of 36.47 +/- 9% in newborn and 49.6 +/- 10% in normal adult controls. These results are discussed as to the status and development of cellular inmunity in human foetus.

Keywords:
Immunity, Adult, Blood Cell Count, Cellular/drug effects, Chemical, Culture Techniques, Fetal Blood/immunology/physiology, Humans, Infant, Lectins/pharmacology, Lymphocyte Activation/drug effects, Microchemistry/methods, Newborn, Stimulation, T-Lymphocytes/immunology/physiology

Authors

R. Ruiz-Requena
F. Garrido
A. Martín-Andrés
C. Osorio


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