New technique for the study of absorption intestinal and data for the best interpretation of the absorption mechanism selective carbohydrates in relation with the phosphatase of intestinal secretion.

Abstract

We propose an original method for the study of intestinal absorption in insolated tracts, ≪in situ≪, which offers perfect conditions for the comparison of results. By means of two canules placed at the tope of the intestinal tract, it is possible to study the absorption of circulating liquids and to accomplish the decreasing of concentration of substances secreted by the mucose. Also it is possible the observation of the absorption capacity in a series of tests in the same tract of the study of the absorption of all types of hydrosoluble matters, and "more specially, of glucids in rats and dogs. The technique in this case it is complet with an original adaptation to the method Folin-copper-colorimetric for the evaluation of sugars.

We explained an original technique for the phosphatases evaluations.

We give a method, as a modification of Hagedorn-Jensen technic, specially indiqated to the sugars absorption experiments. It is more rapid and less inclinated to accidental mistakes.

There are no differences in the intestinal secretion of phosphatase by the stimulating action of selective and unselective sugars.

The circulation of glucose Solutions through the tract decreases slightly the absorption capacity and some more as the circulation takes place more rapidly. If the same liquid is passing several times trough the tract but making previously inactive the secreted phosphatases by means of warm, it is observed a decrease of absorption comparatively to the case of not inactivation.

It is found a partial inhibition of selective absortion of sugars by means of cadmium and phloridzine actions, which act as very, energic inhibitors of phosphatase. A such effect seems originated by actions on the phosphatasic activity in the intestinal content.

The authors have accomplish the acceleration of the glucose absorption, buffered to pH 7 and 6,5 by means of the adition of phosphatase of walls and intestinal secretion.

The best pH for the selective absorption is included between six and seven We confirm the inhibition of the selectivity in rats by cerium, which is demonstrated also in dogs. We attribute this phenomenon, to the precipitation of inorganic phosphate by the cerium in the intestinal content, much better than on cells.

In the intestinal light, after the absortion of glucose, xylose or glicocole, it s found a fraction of organic phosphorus very easy to hydrolyse.

With phosphatase of intestinal secretion we have accomplish the esterification of glucose with phosphates. The maximum esterification in the presence of sodio fluoride and taurocholate, amounts a 25 per cent in 72 hours.

From indicated facts we conclude that the selective absorption must be relationated in some manner with the activity of secreted phosphatases. It is discussed the possibility of hexose phophorilisation in the intestinal content, before penetrating in the cell as the basis of selective absorption.