Quantification by additive RT-PCR of HIV-1 RNA plasma levels in different stages of HIV-1 infection

N. Fernández-Arcás
J.L. Diéguez-Lucena
A.F. Costa
J. García-Villanova
M. Ruiz-Galdón
J. Peña
M. Morell
A. Reyes-Engel
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Abstract

In this study, virion-associated RNA was measured in plasma from twenty six patients in various stages of HIV-1 disease by the additive RT-PCR method. Plasma viral RNA levels were inversely correlated (r = -0.72894) with total CD4+ cell counts and directly (r = 0.86964) with serum titre β2-microglobulin in chronically infected patients. This additive RT-PCR is based on a mathematical logistic adjustment of the standard curve and the use of an internal standard identical to the target molecule, which represents a control system for the efficiency of RT-PCR and allows a continuous assessment of the accuracy based on the recovery.

Keywords:
Hiv-disease, Quantitative pcr

Authors

N. Fernández-Arcás
J.L. Diéguez-Lucena
A.F. Costa
J. García-Villanova
M. Ruiz-Galdón
J. Peña
M. Morell
A. Reyes-Engel


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