Studies on the non-activated and activated forms of the estrogen receptor

J.R. Pasqualini; N. Giambiagi

J.R. Pasqualini

N. Giambiagi

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Published: 1990-03-01

Keywords:

Animals, Antibodies, Binding Sites, Cell Nucleus/metabolism, Cytosol/metabolism, DNA/metabolism, Epitopes/analysis, Estradiol/metabolism, Estrogen/drug effects/metabolism/ultrastructure, Female, Guinea Pigs, Monoclonal/metabolism, Protein Conformation, RNA/pharmacology, Receptors, Ribonucleases/pharmacology, Uterus/analysis/embryology

How to Cite

Studies on the non-activated and activated forms of the estrogen receptor. (1990). Revista Española De Fisiología, 46(1), 1-7. https://revistas.unav.edu/index.php/ref/article/view/46280

47

Abstract

The activation of the steroid receptor is a necessary process for the biological role of the receptor. Many factors are involved in this mechanism; in addition to time, temperature, and salt concentration, RNA and RNAase can also affect the transformation of the non-activated to the activated form of the receptor. Using as a model the estrogen receptor of fetal uterus of guinea-pig, the studies of the interaction with three different monoclonal antibodies (D547, H222 and H226) reveal structural transformation during the process of the receptor activation. These conformational transformations suggest that a change in the exposure of the functional domains of the estrogen receptor occurs during activation.

Keywords:

Animals, Antibodies, Binding Sites, Cell Nucleus/metabolism, Cytosol/metabolism, DNA/metabolism, Epitopes/analysis, Estradiol/metabolism, Estrogen/drug effects/metabolism/ultrastructure, Female, Guinea Pigs, Monoclonal/metabolism, Protein Conformation, RNA/pharmacology, Receptors, Ribonucleases/pharmacology, Uterus/analysis/embryology