Tissue polypeptide antigen liberation and proliferation of MCF-7 cells after synchronization with hydroxytamoxifen and rescue with estradiol

J.M. de Almodóvar
J.D. López-González
R. del Moral
J.C. Fernández
L. Gorgojo
N. Olea
V. Pedraza
72

Abstract

The kinetic of release of the tissular polypeptide antigen (TPA) by MCF-7 cells synchronised by sequential treatment with hydroxytamoxifen (OH-TAM) and 17 beta-estradiol has been studied. The present findings confirm the proliferative effect of estradiol on MCF-7 cells, with a shortening of the doubling time (TD) (22.2 h versus 24.8 h) and an increase in the growing fraction (Fc) (94% versus 81%) when compared with the same parameters measured in cells rescued from OH-TAM but not treated with estradiol. In addition, the action of estradiol was followed by a simultaneous increase in the amount of TPA in the culture medium related with the phases G2/M and G1 of the mitotic cycle. This phenomenon seems to be the reason for the steplike shape of the TPA released curves. The experimental results suggest that in MCF-7 cells the sequential combination of antiestrogenic agents and estrogens leads to a cellular synchronization. Furthermore this synchronization is maintained for at least 3 cycles of cell division.

Keywords:
Antigens/metabolism, Cell Division/drug effects, Cultured/immunology, Estradiol/pharmacology, Humans, Mitosis/drug effects, Peptides/metabolism, Tamoxifen/analogs and derivatives/pharmacology, Tissue Polypeptide Antigen, Tumor Cells

Authors

J.M. de Almodóvar
J.D. López-González
R. del Moral
J.C. Fernández
L. Gorgojo
N. Olea
V. Pedraza


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