Nitrobenzylthioinosine binding cooperativity in chromaffin tissue membranes
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19)
Published:
1992-03-01
Keywords:
Adenosine Triphosphate/metabolism, Adrenal Medulla/metabolism, Animals, Carrier Proteins/antagonists and inhibitors, Kinetics, Membrane Proteins/antagonists and inhibitors, Nucleoside Transport Proteins, Protein Binding, Thioinosine/analogs and derivatives/metabolism
How to Cite
Nitrobenzylthioinosine binding cooperativity in chromaffin tissue membranes. (1992). Revista Española De Fisiología, 48(1), 1-6. https://revistas.unav.edu/index.php/ref/article/view/46371
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Abstract
The nucleoside transporter present in chromaffin tissue membranes has been studied by [3H]nitrobenzylthioinosine (NBTI) binding. This ligand presents a high affinity, with a Kd value of 2.1 +/- 0.2 nM and a Bmax of 1.7 +/- 0.2 pmol/mg protein. From the Scatchard and the semilogarithmic graphical representations a positive cooperativity was deduced, with a Hill coefficient of 1.7 +/- 0.4. In displacement studies of NBTI by the non labelled compound, the Hill coefficient was also higher than 1 (1.44 +/- 0.11) in the presence of ATP. This nucleotide seems necessary to maintain the number of high affinity binding sites.
Keywords:
Adenosine Triphosphate/metabolism, Adrenal Medulla/metabolism, Animals, Carrier Proteins/antagonists and inhibitors, Kinetics, Membrane Proteins/antagonists and inhibitors, Nucleoside Transport Proteins, Protein Binding, Thioinosine/analogs and derivatives/metabolism
Authors
T. Casillas
E.G. Delicado
M.T. Miras-Portugal
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