A rapid and sensitive method for HPLC cholesterol determination in bile

C. Bocos; M. Castro; E. Orozco; J.A. Contreras; E. Herrera

C. Bocos

M. Castro

E. Orozco

J.A. Contreras

E. Herrera

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Published: 1992-09-01

Keywords:

Chromatography, Animals, Bile/chemistry, Cholesterol/analysis, High Pressure Liquid, Ketocholesterols/analysis, Male, Rats, Reproducibility of Results, Sensitivity and Specificity, Sprague-Dawley, Time Factors

How to Cite

A rapid and sensitive method for HPLC cholesterol determination in bile. (1992). Revista Española De Fisiología, 48(3), 211-214. https://revistas.unav.edu/index.php/ref/article/view/46388

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Abstract

A relatively little time consuming simple method based on the treatment of bile with cholesterol oxidase and subsequent high performance liquid chromatography measurement of the 3-ketocholesterol produced in order to determine the level of the cholesterol concentration is described. The method avoids bilirubin interferences, has high reproducibility and recovery assays give 100% values. It is highly sensitive and suitable for use in the determination of cholesterol concentrations in bile and other bilirubin containing biological fluids.

Keywords:

Chromatography, Animals, Bile/chemistry, Cholesterol/analysis, High Pressure Liquid, Ketocholesterols/analysis, Male, Rats, Reproducibility of Results, Sensitivity and Specificity, Sprague-Dawley, Time Factors