Differences in GM-CSF production from mouse peripheral blood and spleen cells stimulated by different lectins

M.C. Alonso
A. Torres
R. Solana
M.R. Manzanares
J.M. García-Castellano
J. Peña
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Abstract

The capacity of mouse peripheral blood and spleen mononuclear cells to produce GM-CSF or CSA, in response to the stimulation by different mitogens (PHA, PWM and ConA) was studied.Each different kind conditioned medium was tested on target bone marrow from BALB/C mice.A significant decrease in the number of CFU-GM, was observed using peripheral blood conditioned medium stimulated by PHA or ConA in comparison with spleen conditioned medium in response to identical mitogens.When PWM is used as source of GM-CSF, significant differences between spleen and blood conditioned media were not observed.The possible significance of these findings is discussed.

Keywords:
Animals, Cells, Colony-Forming Units Assay, Colony-Stimulating Factors/biosynthesis, Culture Media, Cultured, Granulocytes, Inbred BALB C, Lectins/pharmacology, Lymphocytes/drug effects, Macrophages, Mice, Spleen/drug effects

Authors

M.C. Alonso
A. Torres
R. Solana
M.R. Manzanares
J.M. García-Castellano
J. Peña


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