Differences in GM-CSF production from mouse peripheral blood and spleen cells stimulated by different lectins

Abstract

The capacity of mouse peripheral blood and spleen mononuclear cells to produce GM-CSF or CSA, in response to the stimulation by different mitogens (PHA, PWM and ConA) was studied.Each different kind conditioned medium was tested on target bone marrow from BALB/C mice.A significant decrease in the number of CFU-GM, was observed using peripheral blood conditioned medium stimulated by PHA or ConA in comparison with spleen conditioned medium in response to identical mitogens.When PWM is used as source of GM-CSF, significant differences between spleen and blood conditioned media were not observed.The possible significance of these findings is discussed.