4-aminobutyrate-2-ketoglutarate aminotransferase (GABA-T) in human hair follicle

J.A. Armijo
53

Abstract

GABA-T (4-aminobutyrate-2-ketoglutarate aminotransferase) has been found in human hair follicle.Kinetics experiments with hair follicle homogenate supported a ping-pong type of enzymatic mechanism.Extrapolated Km values were 1.02 mmol/l for GABA and 0.45 mmol/l for alpha-ketoglutarate.Hair follicle GABA-T activity was completely inhibited by preincubation of the samples with either 5 x 10(-8) mol/l aminooxyacetic acid or 5 x 10(-4) mol/l gamma-vinyl GABA.The radioenzymatic assay presented is both sensitive enough (only 10 hair follicles are needed for one assay) and economical, making it suitable for clinical practice.Hair follicle GABA-T activity determination could be useful in the study of GABA deficiency diseases (such as epilepsy), congenital GABA-T deficiencies or the control of GABA-T inhibitors treatment.

Keywords:
4-Aminobutyrate Transaminase/analysis/antagonists and inhibitors, Adult, Aminocaproates/pharmacology, Aminooxyacetic Acid/pharmacology, Clinical Enzyme Tests, Hair/enzymology, Humans, Kinetics, Nervous System Diseases/diagnosis, Vigabatrin, gamma-Aminobutyric Acid/metabolism, GABA-transaminase, Hair follicle

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J.A. Armijo


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