4-aminobutyrate-2-ketoglutarate aminotransferase (GABA-T) in human hair follicle

Abstract

GABA-T (4-aminobutyrate-2-ketoglutarate aminotransferase) has been found in human hair follicle.Kinetics experiments with hair follicle homogenate supported a ping-pong type of enzymatic mechanism.Extrapolated Km values were 1.02 mmol/l for GABA and 0.45 mmol/l for alpha-ketoglutarate.Hair follicle GABA-T activity was completely inhibited by preincubation of the samples with either 5 x 10(-8) mol/l aminooxyacetic acid or 5 x 10(-4) mol/l gamma-vinyl GABA.The radioenzymatic assay presented is both sensitive enough (only 10 hair follicles are needed for one assay) and economical, making it suitable for clinical practice.Hair follicle GABA-T activity determination could be useful in the study of GABA deficiency diseases (such as epilepsy), congenital GABA-T deficiencies or the control of GABA-T inhibitors treatment.