Binding and antilipolytic action of insulin in isolated adipocytes from cortisol-treated rats

C. Calle
P. Sánchez-Casas
M.C. Carranza
M.A. Simon
P. Mayor
72

Abstract

The effects of an in vivo cortisol-treatment to rats (2 X 2 mg/rat/day, for one week) on insulin plasma levels, insulin binding and antilipolytic activity in rat adipose tissue were investigated.Hyperinsulinemia together with an increase in insulin degradation in the serum of cortisol-treated rats were observed.The adipocytes from cortisol-treated animals showed a statistically significant decrease in insulin binding but no change in receptor numbers [cortisol-treated 103,000 +/- 8,000 (n = 8) receptors/cell and controls 138,000 +/- 15,000 (n = 16) receptors/cell], together with unchanged receptor affinity [ED50: cortisol-treated 3 X 10(-09) M and controls 3.2 X 10(-09) M], and a decreased sensitivity to the antilipolytic effect of insulin.The evidence presented for pre-receptor, receptor and post-receptor insulin defects on the action of cortisol in isolated rat adipocytes could represent a coordinated mechanism by which cortisol exerts "insulin resistance" in this tissue.

Keywords:
Adipose Tissue/cytology/drug effects, Animals, Glycerol/metabolism, Hydrocortisone/pharmacology, Inbred Strains, Insulin/blood/metabolism, Insulin/drug effects/metabolism, Lipolysis/drug effects, Male, Rats, Receptor

Authors

C. Calle
P. Sánchez-Casas
M.C. Carranza
M.A. Simon
P. Mayor


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