Development of a fast solid-phase enzyme immunoassay for C-reactive protein

Abstract

A fast sandwich enzyme immunoassay has been developed for C-reactive protein (CRP).This method can be used for screening CRP concentration in large numbers of samples providing a non precipitation, non agglutination and non radioactive alternative for assessment of human CRP.Advantages over previously reported CRP sandwich assays include: assay time was reduced from 4 1/2 h to 45 min, incubations were made at room temperature instead of 37 degrees C and serum dilutions required were 100-400 fold instead of 10000-20000 fold.Correlations were good with both nephelometry and phosphorylethanolamine binding assay.The 45% false positives found with the slide-latex anti C-reactive protein method were reduced to 0% by the use of the described method.