Liver phospholipid metabolism in Gallus domesticus

Abstract

A method is described for the extraction and thin-layer chromatographic separation of phospholipids.Eight separate phospholipid fractions are easily distinguishable.Liver phospholipid metabolism in Gallus domesticus has been studied in newborn chicken (less than 24 h old).(1-14C) serine, 3-14C) serine, (2-14C) ethanolamine and (methyl-14C) choline were used as radioactive precursors.Serine is incorporated as lysophosphatidylserine, ethanolamine as phosphatidylethanolamine, and choline as L-alpha-glycerophosphorylcholine.The observed pathways for serine and ethanolamine incorporation and turnover are the same as those described for mammals; the main pathway for choline is the synthesis of phosphatidylcholine from lysophosphatidylcholine.