Proteolytic susceptibility of alpha-amylase of the pancreas of swine deprived of its structural calcium

J.C. Milicua
F.A. García
J.M. Macarulla
62

Abstract

Hog pancreas alpha-amylase (alpha-1-4-glucan-glucan hydrolase, E.C.3.2.1.1) lost its structural calcium by action of EDTA at 20 degrees C.Enzymatic activity experimented a decrease whereas a big increase in proteolytic susceptibility to bovine pancreas trypsin (E.C.3.4.4.4) was shown.Native alpha-amylase had an activity of 2,730 mg maltose/min X mg enzyme and a Km of 0.222% amylose, the activity of calcium depleted amylase being of 1,640 mg maltose/min X mg enzyme and Km 0.571% amylose.Simple methods for evaluating proteolytic susceptibility of alpha-amylase micro-amounts against trypsin action, and for the measurement of alpha-amylase activity in polyacrylamide rod gels were also described.

Keywords:
Animals, Apoenzymes/metabolism, Apoproteins/metabolism, Calcium/metabolism, Edetic Acid/administration and dosage, Hydrolysis, Kinetics, Pancreas/enzymology, Swine, alpha-Amylases/metabolism

Authors

J.C. Milicua
F.A. García
J.M. Macarulla


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