Method of assay for 4-aminobutyrate-2-oxoglutarate aminotransferase

M.P. González
S. Cañadas
F. Muñoz
J. Sánchez-Prieto
V. Morales
42

Abstract

In this paper an analytical colorimetric method is presented where 2,4-dinitrophenyl hydrazine is used to measure small amounts of succinic semialdehyde in the presence of 2-oxoglutarate.This method is applicable to the measurement of 4-aminobutyrate-2-oxoglutarate aminotransferase activity, where succinic semialdehyde formed during the enzymatic reaction has to be measured in the presence of 2-oxoglutarate.

Keywords:
4-Aminobutyrate Transaminase/analysis, Animals, Colorimetry, Ketoglutaric Acids/metabolism, Phenylhydrazines, Rats, gamma-Aminobutyric Acid/analogs and derivatives/analysis

Authors

M.P. González
S. Cañadas
F. Muñoz
J. Sánchez-Prieto
V. Morales


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