Metabolic studies with 65Zn. X. Biosynthesis of alkaline phosphatase in cultures of Pseudomonas fluorescens

V. Morales; P. Díaz-Rubio; C. del Amo; A. Santos-Ruiz

V. Morales

P. Díaz-Rubio

C. del Amo

A. Santos-Ruiz

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Published: 1975-03-01

Keywords:

Alkaline Phosphatase/biosynthesis, Hot Temperature, Magnesium, Pseudomonas fluorescens/enzymology, Zinc Radioisotopes/metabolism

How to Cite

Metabolic studies with 65Zn. X. Biosynthesis of alkaline phosphatase in cultures of Pseudomonas fluorescens. (1975). Revista Española De Fisiología, 31(1), 9-14. https://revistas.unav.edu/index.php/ref/article/view/49448

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Abstract

Alkaline phosphatase from Pseudomonas fluorescens has been partially purified. Labelled 65ZnCl2 in the culture medium is incorporated in the most purified preparations. The enzyme shows a pH optimum of 7.2-7.5 and a Km value for p-nitrophenyl phosphate of 1 times 10(-4). Zinc ions at high concentration inhibit enzyme activity. The protective effect of Mg on the inactivation of alkaline phosphatase by heat is also reported.

Keywords:

Alkaline Phosphatase/biosynthesis, Hot Temperature, Magnesium, Pseudomonas fluorescens/enzymology, Zinc Radioisotopes/metabolism