Influence of the sporulation on glucose-06-phosphate dehydrogenase activity from Aspergillus oryzae (Ahlburg)

Abstract
Both the specific and the total activity of glucose-06-phosphate dehydrogenase (E.C.1.1.1.49) of mycelium of Aspergillus oryzae decline progressively from day 6th of incubation, when the period of sporulation begins.Total cellular protein decreases parallely.Cicloheximide stops protein synthesis but not the inactivation of glucose-06-phosphate dehydrogenase.Inhibitors of proteolytic enzymes do not prevent the loss of enzyme activity when added to the cultures.Experiments show no inhibitors of the enzyme to be present in late-stage cells.The enzyme is not excreted into the culture medium.
Keywords:
Aspergillus oryzae/enzymology, Aspergillus/enzymology, Bacterial, Glucosephosphate Dehydrogenase/metabolism, Spores, Time Factors
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