Effects of pepstatin A on neutrophils; cross-deactivation with FMLP

Abstract

The ability of pepstatin A, a protease inhibitor produced by Streptomyces testaceus, to elicit a number of responses by the human PMN has been studied.In lysozyme and beta-glucuronidase release, pepstatin A 10(-5)M is equivalent to the synthetic oligopeptide N-formyl-methionyl-leucyl-phenylalanine (FMLP) 10(-7)M.In superoxide release, pepstatin A 10(-5)M produces 80% of that originated by FMLP 10(-7).After two minutes of incubation the superoxide release is important, there being no further increase after 10 minutes.Preincubation of the cells with cytochalasin B before stimulation with pepstatin A elicits a noticeable increase in O2- release.In chemotaxis, pepstatin A 10(-06) originates the same cell motility as FMLP 10(-09).Pepstatin A produces a cross deactivation with FMLP which adds further evidence to the hypothesis that both stimuli compete for the same receptor in the PMN.