Changes in the metabolism of muscle RNA in rats given a high protein diet

Abstract

The effect of a high protein diet (20% casein + D,L-methionine) administered to adult Wistar rats on some aspects of muscle RNA metabolism has been studied.Body weight increased in spite of lower intake.However, gastrocnemius muscle remained unmodified, although protein content increased.Total RNA decreased in the whole muscle although RNA/DNA ratio did not change.Protein synthesis capacity diminished 81% relative to controls in spite the fact that an excessive amount of available amino acids exists.RNA loss might depend on a high catabolism, since acid RNase activity increased over control values.Therefore, it may be concluded that a high protein diet leads to a lower protein synthesis capacity through an elevated RNA breakdown.