Peroxidase and IAA oxidase in germinating seeds of Cicer arietinum L

Abstract

Peroxidase and indole acetic oxidase (IA oxidase) activities in seeds of Cicer arietinum L.were studied at 72 h of germination.Both activities behaved differently in relation to optimum pH, stability against temperature and time of storage of 4 degrees C.Disc electrophoresis on polyacrilamide gel showed the existence of five anionic and three cationic isoenzymes with peroxidase activity by using benzidine as substrate; three of the anionic isoenzymes and one cationic isoenzyme possessed a simultaneous IAA oxidase activity as well.