Positive selection by panning of B lymphocytes using unfractionated anti-immunoglobulin antiserum

Abstract
A simple method allowing a positive selection of mouse B lymphocytes by "panning" has been achieved using rabbit anti-mouse immunoglobulin antisera without further purification.Plastic petri dishes were coated with the gamma-globulin fraction of normal mouse serum and then incubated with excess rabbit anti-mouse gamma globulin antiserum.These plates were then used for the selection of B lymphocytes by incubation of spleen cells in the plates.The adherent cells wee above 95% B lymphocytes as judged by immunofluorescence staining, proliferated in response to bacterial lipopolysaccharide and were not stimulated by concanavalin A.
Keywords:
Cell Separation, Immunosorbent Techniques, Animals, Anti-Idiotypic/immunology, Antibodies, Antigen, B-Cell/immunology, B-Lymphocytes/immunology, Concanavalin A/pharmacology, Female, Immune Sera, Inbred BALB C/immunology, Inbred C57BL/immunology, Lipopolysaccharides/pharmacology, Lymphocyte Activation, Mice, Receptors, «Panning», B lymphocytes, Positive selection
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