Neurons from rat forebrain and cerebellum.Isolation and biochemical characterization

Abstract

Isolation of neurons from rat forebrain and cerebellum has been performed by a method including trypsin incubation and tissue disaggregation by filtration through successive nylon meshes with a different pore size.Phase contrast microscopy shows highly purified cell preparations.Lactate dehydrogenase, acetylcholinesterase and (Na+-K+) ATPase activities indicate that neurons possess a high cytosolic content and show a good preservation of the plasmatic membrane.