Personal experience with the technic of estimation of the antidiuretic powder of body fluids

Abstract

The antidiuretic power of urine and of organic humours is being measured according to the following technique:

1) 150 c.c. of the problem liquid are collected to which are added 5 c.c. of inerthiolale sodium at 1/1.000 and 2 c.c. of acetic acid. This liquid is placed in a dialyzator, tlie bottoni of which has been previously closcd up with a celoplian membraue, and both are placed into a crystallizing vessel containing distilled water (which is chan- ged at half-time of the test). The stove and ventilator are set in motion and are conveniently placed so that the current air of the latter is heated by the fonner to some 20°C. At the same time the food is withdrawn from the two groups of four rats each, chosen for the experiment.

2) Ten hours after the mentioned preparations have been initiated, hydration of the rats is proceeded with by means of a gastrie injection of water in a quantity of 2,5 % of their weight.

3) Two hours after the fonner operaticn, the dialysis-concentration being .terminated, the intraperitoneal injection of the prepared problem-liquid is proceeded with, same being distributed exactly among the eight rats experimented upon. Definitive hydratation follows in a quantity corresponding to 5 % of their weight.
The rats are placed in two groups of four animals each in a corresponding cage, their diuresis being measured every 15 minutes, after rejection of the first determination.

4) The result in «time of diuresis» is noted, viz : the time of the test necessary for the rats to eliminate a quantity of urine equivalent to 2,5 % of their weight.

5) Means of security : a) Observation of diuresis in the rats in the interval between the first and the second hydration. b) Measurement of the density in doubthful cases. We shall expose the nesults obtained expressed in «time of diure­ sis» in the multiple experimenta realized with our technique instandard tests in order to precise the times of normal diuresis : Standard Test:

1) Ingestion of water by the digestive way. Times of diuresis comprised between 50 and 70 minutes.

2) Water by the digestive way, plus intraperitoneal injection of 2 c.c. of physiological serum.

3) Water by the digestive way, plus intraperitoneal injection of 2 c.c. of physiological serum. Time of diuresis comprised betwcen 65 and 85 minutes.

4) Water by the digestive way, plus intraperitoneal injection of dialyzed and concentrated uriníƒe of a normal individual. Times of diuresis comprised betwcen 70 and 100 minutes.

5) Water by the digestive way plus hypophysine by the intraperi­ toneal way. a) less than 1/100 unities Voegtin per rat, Time of diuresis less than 100 minutes, b) more than 1/100 unities Voegtin per rat, Time of diuresis «almost always» more than 100 minutes.

Time roughly proportional to the quantity of hypophysine injected : With quantities only slightly surpassing the nuniber of 1/100 U.V. per rat, thc time of diuresis lias been on some occasions slightly inferior to 100 minutes.

The authors conclude that a time of diuresis superior to 100 mi­nutes indicates a pathologically increased antidiuretic power in the test liquid, whilst a time of diuresis inferior to 50 minutes indicates a diuretic power of the said test liquid.