Improvements in the cultivation of plant tissues

J.L. García-Bilbao
J.M. Garrido
46

Abstract




The aseptic conservation of Plant Tissue Cultures perfor- med in Erlenmeyer flasks stoppered with cotton in humid en- vironments was lost by the development of Filamentous Fungi through the stopper.


Sorbic acid, as fungistaticagent, impregnating the cot­ ton prevenís the microbial spread ;but the humid cotton redu­ces the access of air.


Glass-wool, laterally protected with filature of asbestos, enable to fíame, to avoid the effeets of external spores, and allowing a good aeration constitutes a practical stopper.




Keywords:
Immunologic Tests, PLANTS, Plants, Research Design, TISSUE CULTURE, Tissue Culture Techniques

Authors

J.L. García-Bilbao
J.M. Garrido


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