Ionization capacity of versenate calcium disodium

Abstract

In the present work the ionization capacity of calcium-di- sodic-elhilendiaminotetracetate (Ca-Na,-EDTA) is studied, aspect we consider fundamental in judging its valué as a recalcifier. For it we have used a new technique owed to Soulier and col. (4), based on the utilization of a coagula­ ron system formed by magnesied amberlite or R.A.M., diluted thromboplastin solution and the problem serum inactivated with 60°C heat during 30 minutes.

This problem se­ rum was obtained from rabbits previously injected with 100 mg of Ca-Na2-EDTA per kg of weight. The lowering of the coagulation time of the system is, by this method, proportional to the amount of ionic calcium present in the se­ rum which is tested.

From our experiences (see figure) it is deduced that, after 60 minutes, Ca-Na2-EDTA liberates, in the organism, an amount of ionic calcium equivalent to 7-16 % of the total calcium contained in its molecule. In spite of this positivoresult, the remineralizing power of Ca-Na2-EDTA is doubted, due to its metal chelating condition.