In vitro production of 14CO2 from D-glucose-06-C14 in different areas of the hypothalamus, limbic system and pituitary gland during different stages of the estrus cycle of the female rat

O. Schiaffini
E. Herrera
A. Gallego
46

Abstract




In vitro formation of “COj was determined in slices of anterior, middle, posterior hypotha- lamic areas, amygdala, hippocampus and pituitary of rats during oestrus and dioestrus. Glucose-l-C** and glucose-6-C“, were used as substratos.
The result show that formation of UCO, from glucose-6-C1* by anterior and posterior hypothalamic areas increase in rats during oestrus as compared with animal in dioestrus. The results are statistically significan! (p < 0.02 anterior hypothalamus; p < 0.05 posterior hypo­ thalamus).


When glucose-l-C1* was used as a substrate no significant differences were observed.


The quotient CO, formation from glucose-l-C** and CO3 formation from glucose-6-C1* decreases in the different hypothalamic areas and amygdala from rats in oestrus as compared with animáis in dioestrus.




Keywords:
Estrus, Animals, Carbon Dioxide/metabolism, Carbon Isotopes, Female, Glucose/metabolism, Hypothalamus/metabolism, Limbic System/metabolism, Pituitary Gland/metabolism, Pregnancy, Rats

Authors

O. Schiaffini
E. Herrera
A. Gallego


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