Extracorporeal hepatic perfusion with isolated dog liver

Abstract

Extracorporeal liver perfusion was carried out in 36 experiments. The isolated dog liver was used. Liver function during the experiments was measured with three para­ meters: B.S.F. clearance, oxigen consumption and biliary output. Six different types of perfusion modifications were introduced. At the end of each experiment, the microscopic appearance of the liver was studied.
The results showed that the viability of the dog liver under our conditions of per­fusion was 60-120 minutes. If the period of cold ischemia was avoided, the viability was prolongued to 240-300 minutes.