Determination of serum testosterone using radioimmunoanalysis without previous extraction

E. Ortega
E. Ruiz
B. Neira
C. Osorio
77

Abstract

A modification to the radiochemical assay method to measure testosterone in serum, that renders unnecessary the extraction of testosterone from a serum sample is described. The serum is heated at 60 degrees C for 1 hour, thereby testosterone binding proteins are destroyed, then radioimmunoassay follows as usual.

Keywords:
Humans, Radioimmunoassay/methods, Testosterone/blood

Authors

E. Ortega
E. Ruiz
B. Neira
C. Osorio


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