Nitrate reductase from Spinacea oleracea. FAD and the reactivation of the enzyme treated with p-Hydroxymercuribenzoate
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20)
Published:
1977-12-01
Keywords:
Dihydrolipoamide Dehydrogenase/analysis, Enzyme Reactivators, Flavin-Adenine Dinucleotide/analysis, Mercuribenzoates/pharmacology, Nitrate Reductases/analysis, Vegetables/analysis
How to Cite
Nitrate reductase from Spinacea oleracea. FAD and the reactivation of the enzyme treated with p-Hydroxymercuribenzoate. (1977). Revista Española De Fisiología, 33(4), 287-290. https://revistas.unav.edu/index.php/ref/article/view/49536
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Abstract
Spinach nitrate reductase complex previously inactivated by treatment with mercurials p-hydroxymercuribenzoate or p-hydroxymercuriphenyl sulphonate can be reactivated by incubation with dithioerythritol. The reactivation of NADH-diaphorase seems to be FAD-dependent, whereas that of FNH2-nitrate reductase is not. The requirement of FAD for NADH-inactivation of nitrate reductase treated with p-hydroxymercuribenzoate disappears after treatment with dithioerythritol.
Keywords:
Dihydrolipoamide Dehydrogenase/analysis, Enzyme Reactivators, Flavin-Adenine Dinucleotide/analysis, Mercuribenzoates/pharmacology, Nitrate Reductases/analysis, Vegetables/analysis
Authors
F. Castillo
F.F. de la Rosa
E. Palacián
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