Study of a set of chromatographic parameters for the determination of estrogens

M.E. Campos; J.A. Gómez-Capilla; C. Osorio

M.E. Campos

J.A. Gómez-Capilla

C. Osorio

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Published: 1978-03-01

Keywords:

Chromatography, Estrogens/isolation and purification, Female, Gas, Humans, Liquid, Pregnancy

How to Cite

Study of a set of chromatographic parameters for the determination of estrogens. (1978). Revista Española De Fisiología, 34(1), 21-24. https://revistas.unav.edu/index.php/ref/article/view/49616

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Abstract

Optimum chromatographic parameters for the separation and detection of estrogen TMS derivatives are described. Estrogens are best separated by using a 2 m glass column with 1% SE-30 load; an 8' iso temperature program, 230 degrees C, 3 degrees C/min, 296 degrees C; 24 ml/min nitrogen flow; 33 ml/min hydrogen flow and 1.5 Kg/cm2 air pressure.

Keywords:

Chromatography, Estrogens/isolation and purification, Female, Gas, Humans, Liquid, Pregnancy