Gas-liquid chromatography determination of neutral monosaccharides from a human urine glycopeptide

Abstract

Optimum conditions were determined for the satisfactory separation of the carbohydrate residues found in glycoproteins or glycopeptides by using silica gel column chromatography of monosaccharides as their trimethylsilyl ethers. The method is suitable for fractionation of sugar mixtures into neutral sugars and aminosugars. Chromatography on silica and gas-liquid chromatography were used in succession for the rapid determination of the neutral monosaccharides from a human urine glycopeptide.