Convenient Procedures for Isolating the Mitochondrial Fraction Suitable for the Study of Mitochondrial Nucleic Acids from Normal and Neoplastic Cells

D. Guerritore; A. Frigola; E. Piccolella; A. Montalvo

D. Guerritore

A. Frigola

E. Piccolella

A. Montalvo

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Published: 1975-07-01

How to Cite

Convenient Procedures for Isolating the Mitochondrial Fraction Suitable for the Study of Mitochondrial Nucleic Acids from Normal and Neoplastic Cells. (1975). Revista Española De Fisiología, 31(3), 191-196. https://revistas.unav.edu/index.php/ref/article/view/50302

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Abstract

A useful procedure for the large scale preparation of mitochondria from normal and neoplastic tissues is described using mannitol, sucrose, EGTA and bovine serum albumin. It overcomes satisfactorily the difficulties involved in the great lability of tumour cell mitochondria. No significant nuclear, cytoplasmic, lysosomal or bacterial contamination was present.

Authors

D. Guerritore

Laboratory of General Pathology, Faculty of Sciences, University of Rome, Istituto Regina Elena, Rome, and Institute of Biological Chemistry, University of Bari (Italy)

https://orcid.org/0000-0002-1825-0097

A. Frigola

Laboratory of General Pathology, Faculty of Sciences, University of Rome, Istituto Regina Elena, Rome, and Institute of Biological Chemistry, University of Bari (Italy)

https://orcid.org/0000-0002-1825-0097

E. Piccolella

Laboratory of General Pathology, Faculty of Sciences, University of Rome, Istituto Regina Elena, Rome, and Institute of Biological Chemistry, University of Bari (Italy)

https://orcid.org/0000-0002-1825-0097

A. Montalvo

Department of Biochemistry, Faculty of Medicine, University of Santander, Santander (Spain)

https://orcid.org/0000-0002-1825-0097