Intestinal secretion of phosphatases

Abstract

We study the phosphatasic intestinal secretion in several laboratory animals, principally in guinea pigs.

By means of stimulation of nutritive matters present on the intestinal content, occurs a Stark phosphatasic secretion by mucose cells. This fact is observed with the ordinary mixed aliments and several hexoses, xylose, glicocole, peptone, glicerophosphate, oleic acid and nucleinic acid. In the same way on the intact animal (gastric sond) than in isolated intestinal loops ≪in situ≪. It consists in a local Chemical stimulation, very sparringly specific: it does not exist appreciable differences between the provocated secretions by the different substances employed, although then increased with their concentration on the intestinal content. The quantity of the provocated secretion by isotonic solutions of glucose in an hour amounts approximately the twenticth part of total phosphatase of the intestinal mucose.

The mucose conteni on phosphatases, the secretion of this ferment and the selective absorption of sugars decreased propoftionally though the long of intestine. In the colon no phosphatasie secretion exists.

We study the variation of phosphatasie richese of the intestinal content during the digestion and formation of feces.

The guinea pig phoetus nearer to the end of developement, havent just the phosphatase in their intestinal content and the concentration of the ferment on the mucose is of a similar order which corresponds to the adult.

The most favourable pH of the secreted phosphatase is of 9,4 : to a different manner of the mucose phosphatase presents the first at acid pH a little predominant sintetical activity.

We study the stimulating or inhibitory action of magnesium, urethane, florricine, glicocolle, taurocolate, cianur, magnese, fluoruro. Ni, Cu, U, Cd, Co and Sr on the mucose and secreted phosphatases. The magnesium activation on this ones is less important than on the other ones, which permits to sospect a duality of alkaline phosphomonoesterase of intestine.

We evidence apecially the syntetical action of secreted phosphatases and the activation by means of taurocolate, all of this at a not very acid pH in relation with his possible physiological signification.