R5020 enhances PGE2 stimulated steroidogenesis in cultured rat granulosa cells

L.F. Fanjul
J. González
P. Santana
F. Estévez
J. Quintana
C.M. de Galarreta
60

Abstract

Progesterone biosynthesis and metabolization to 20 alpha-hydroxyprogesterone was stimulated in granulosa cells cultured in the presence of 20 ng/ml of follicle stimulating hormone (FSH) or increasing concentrations of PGE2 (10(-9)-10(-7)M). Concurrent treatment with the synthetic progestin R5020 (10(-6) M) enhanced the FSH or PGE2 stimulated progesterone and 20 alpha-hydroxyprogesterone accumulation in culture media, as well as delta 5-3 beta-hydroxysteroid dehydrogenase activity in granulosa cell homogenates. These findings may represent another example of an autocrine control mechanism in which the steroidogenic product of the granulosa cell exerts an ultra-short loop regulation of its own production.

Keywords:
20-alpha-Dihydroprogesterone/biosynthesis, 3-Hydroxysteroid Dehydrogenases/metabolism, Animals, Cells, Chemical, Cultured, Dinoprostone/pharmacology, Drug Synergism, Female, Follicle Stimulating Hormone/pharmacology, Granulosa Cells/drug effects/metabolism, Progesterone/biosynthesis, Promegestone/pharmacology, Rats, Sprague-Dawley, Stimulation

Authors

L.F. Fanjul
J. González
P. Santana
F. Estévez
J. Quintana
C.M. de Galarreta


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