Perfusion of the dog tibia isolated for the study of mineral metabolism

M. Babé
P. Esbrit
M.L. Traba
C. Castilla
A. Rapado
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Abstract

A method for perfusion of isolated canine tibia is described.With this procedure the influence of different albumins on the uptake of tritiated 25-hydroxyvitamin D3 (3H-25(OH)D3) by the tibia has been studied.Bovine serum albumin at 1% concentration seems to be optimum for this system.This experimental approach seems to be useful for the study of mineral metabolism.

Keywords:
Animals, Calcifediol/metabolism, Dogs, Perfusion/methods, Serum Albumin/metabolism, Tibia/metabolism

Authors

M. Babé
P. Esbrit
M.L. Traba
C. Castilla
A. Rapado


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