Transmural potential differences and short-circuit current intensity in the posterior intestine of Blennius parvicornis

Abstract

Simultaneous measurements of the transmural potential difference (PD) and the short-circuit current intensity (Isc) in the posterior intestine of the fish Blennius parvicornis were made in normal Ringer and in solutions of different ionic composition.The ouabain effects on these two parameters were also tested in normal Ringer solution.The absence of K+ from the Ringer solution on both the mucosal and serosal sides has no apparent effect on the PD and Isc within the first 15 min, but it makes them null after 30 min.When Na+ is substituted in both compartments, using Tris as substitute, a serosal negativity increase is initially observed, but it gradually decreases to zero after 30 min of experimentation.Similarly the PD and Isc drop to zero in the absence of Cl- (sulfate as substitute).Ouabain diminishes the serosa negative potential difference to zero after 30 min presenting a lineal relation to the Isc.A likely transport mechanism for Cl- dependent on the Na+ - K+ pump, is discussed.