Quantification of lectin receptors on B, T, T-gamma, and T-mu lymphocytes.II.PHA, SBA, Dolichos biflorus, and Tetragonolobus purpureus

Abstract

Plasma membranes composition and structure of B, T, T gamma and T mu lymphocytes from peripheral blood have been studied using four 125I-labelled lectins.The results showed that B lymphocytes have a greater number of receptors for SBA and Dolichos biflorus lectins than T lymphocytes, whilst the opposite was observed for PHA-lectin.However, no significant differences between the two lymphocyte populations were found regarding the receptors for Tetragonolobus purpureus lectin.Among T lymphocyte subpopulations, SBA, Dolichos biflorus and tetragonolobus purpureus lectins appeared to have higher specificity for T gamma lymphocytes and PHA for T mu.PHA-lectin had greater affinity for B lymphocytes, while the rest showed higher affinity for T lymphocytes.The T gamma subpopulation had lower association constant for PHA-lectin than T mu lymphocytes, whilst the contrary was found to be true for the other three lectins.