Separation of B, T, T-gamma and T-mu lymphocytes using rosettes of various types of erythrocytes and polyacrylamide.Comparative study

Abstract

The present work compares the effectiveness of, both, red blood cells from different species (sheep, ox and chicken) and polyacrylamide beads to form rosettes to separate B, T, T gamma and T mu lymphocytes.Ox red cells showed a scanty agglutination, so that they appeared to be optimal to isolate B, T gamma and T mu lymphocytes by EA rosettes.Sheep red cells were the best to separate both T and B lymphocytes by spontaneous and EAC rosettes respectively; on the other hand, chicken red blood cells, which showed a high capacity to form rosettes, were not useful to separate lymphocytes, as it was difficult to separate them from the lymphocytes.Polyacrylamide beads, with bound specific antibody against surface immunoglobulins, proved to be a good method for B lymphocyte identification, but not for lymphocyte isolation, since it was difficult to eliminate the polyacrylamide.