Regulation of glycolysis in sea bass liver: phosphofructokinase isozymes

M.D. Fideu; M.L. Pérez; M.J. Herranz; M. Ruiz-Amil

M.D. Fideu

M.L. Pérez

M.J. Herranz

M. Ruiz-Amil

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Published: 1989-06-01

Keywords:

Glycolysis, Allosteric Regulation, Animals, Bass/metabolism, Fructosephosphates/metabolism, Isoenzymes/isolation and purification/metabolism, Kinetics, Liver/enzymology, Molecular Weight, Perciformes/metabolism, Phosphofructokinase-1/isolation and purification/metabolism, Temperature, Sea bass, Liver, Isozymes PFK

How to Cite

Regulation of glycolysis in sea bass liver: phosphofructokinase isozymes. (1989). Revista Española De Fisiología, 45(2), 179-186. https://revistas.unav.edu/index.php/ref/article/view/46823

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Abstract

Sea bass (Dicentrarchus labrax L.) liver phosphofructokinase (PFK) presents biphasic kinetics with respect to fructose-06-phosphate (F-06-P) in experiments carried out with crude extract.After the enzyme had been purified, two isozymes have been detected after chromatographic treatment.The two isozymes present different kinetic behaviour PFK-L1, the first eluted phosphofructokinase activity shows positive cooperativity with respect to fructose-06-phosphate and PFK-L2, the second activity fraction, has a Hill coefficient of 0.38 (negative cooperativity).The first isozyme shows less affinity for fructose-06-phosphate than that shown by PFK-L2.The joint kinetics of both isozymes produces a biphasic kinetics with respect to fructose-06-phosphate, similar to that observed in crude extracts.

Keywords:

Glycolysis, Allosteric Regulation, Animals, Bass/metabolism, Fructosephosphates/metabolism, Isoenzymes/isolation and purification/metabolism, Kinetics, Liver/enzymology, Molecular Weight, Perciformes/metabolism, Phosphofructokinase-1/isolation and purification/metabolism, Temperature, Sea bass, Liver, Isozymes PFK