Mitochondrial heterogeneity in Aspergillus nidulans: in vivo protein biosynthetic activities of the mitochondrial populations

J. Montoya
M.J. López-Pérez
38

Abstract

Two mitochondrial populations with cytochrome c oxidase activity, and densities of 1.07 and 1.08 g/ml have been separated by means of a dextran-sucrose gradient.The highest enzymatic specific activity appeared in the 1.07 g/ml band.The mitochondria assayed by their in vivo cycloheximide resistant leucine incorporation appeared at the 1.08 g/ml density band of the gradient.Some amount of activity of cytochrome c oxidase, and leucine incorporation appeared at the bottom of the gradient.The cytochrome spectra in liquid nitrogen of each band showed different proportion of cytochrome(s) a, b and c.The highest ratio of cytochrome a and cytochrome b in respect to cytochrome c appeared in the 1.08 g/ml band.

Keywords:
Protein Biosynthesis, Active, Aspergillus nidulans/cytology, Biological Transport, Cycloheximide/pharmacology, Cytochromes/analysis, Electron Transport Complex IV/analysis, Leucine/metabolism, Mitochondria/drug effects/enzymology/metabolism

Authors

J. Montoya
M.J. López-Pérez


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