Isocitrate lyase activities in Pinus pinea seeds

J. Montoya-Villarroya
M.J. López-Pérez
43

Abstract

Some features of cytoplasmic and glyoxysomal isocitrate lyase obtained from stratified and germinated P. pinea seeds, respectively, are described. Both activities showed a different electrophoretic mobility on cellulose acetate and elution behaviour on Sephadex G-200. Cycloheximide inhibited the increase of both activities during the first days of germination. Chloramphenicol did not show the same effect.

Keywords:
Oxo-Acid-Lyases/isolation and purification, Trees, Cellulose Acetate, Chloramphenicol/pharmacology, Chromatography, Cycloheximide/pharmacology, Cytoplasm/enzymology, Electrophoresis, Gel, Isocitrates, Seeds/enzymology

Authors

J. Montoya-Villarroya
M.J. López-Pérez


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