In vivo heat damage to the E.coli nucleoid

Abstract
The effect of heat damage in vivo on the tertiary structure of E. coli folded chromosome or nucleoid was studied by sedimentation in neutral sucrose gradients. The heat treatment produced an increase in the amount of protein associated with the nucleoid together with an unfolding of the nucleoid structure as determined by the increase in speed dependence.
Keywords:
Hot Temperature, Bacterial/metabolism, Centrifugation, Chromosomes, Density Gradient, Escherichia coli/genetics, Macromolecular Substances, Nucleic Acid Conformation
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