Heat damage and repair in the Escherichia coli nucleoid: kinetics based on sedimentation analysis

Abstract

Changes in the structure of the Escherichia coli nucleoid during heat damage and repair were followed by sedimentation analysis in neutral sucrose gradients.Heating at 50 degrees C results first in a decrease in the sedimentation coefficient of the isolated nucleoid.Increasing the heating time, a subsequent increase in sedimentation coefficient is observed.After a heat shock (i.e.4 min at 50 degrees C), a short incubation at 25 degrees C (i.e.5 min) allows the nucleoid to repair and return to the sedimentation coefficient of control unheated nucleoids.The nucleoids heated at 50 degrees C for longer periods and incubated afterwards at 25 degrees C demonstrate a different pattern of structural repair.They associate with protein in the first stage of the repair period.