Purification of fibronectin using gelatin derivatives

Abstract

This work compares two distinct methods, column and batch, for the purification of fibronectin, from different plasma fractions, using gelatin and one of its derivates (Hemoce, Behring).The yields of both techniques at quantitative as well as qualitative levels (levels of immunologically active fibronectin), are evaluated.The data indicate that better conservation of immunological immunological characteristics is obtained with the use of gelatin derivatives (Hemoce).The plasma fraction does not have significant influence on the process yields.