Curves of fractionation with sodium sulfite and hyposulfite of blood proteins. I. Normal human serums

Abstract

In this work we study the fractioning curve with sodium sul­phite and hyposulphite of normal human serums. Twelve serums have been fractionated with sodium sulphite and twelve with hy­ posulphite and the average curve of this is given.

With sodium sulphite the curve sliows two angles with concentrations of 19,2 and 25,9 g. percent. We have compared these results with those obtained by the investigators wlio have studied the fractioning by salts and by the electrophoresis.

With sodium hyposulphite we obtained three angles with concentrations of 30,72, 35,52 and 42,24 ; judging by the solubility va­lues on the ordinate axis it is shown that the two last correspond with the forementioned of the sulphite. The first, i.e. the concentration 30,72 % docs not offer an equivalcnt in the curve of sulphite. By solubility values it would correspond to a concentration of sodium sulphite or sulphate of 16,32 g. per cent. From the angles found in the hyposulphite curve and from the results of the investigators who have studied comparatively the salt fractioning and the electrophoresis, it is to be sucggested that these angles would corres­ pond with the fractioning of the albumin and the a, 13, and y globulins obtained by electrophoresis.

It is therefore considercd more recomendable from the theoretic point of view use for fractioning the sodium hyposulphite, as to from a practical point of view, because its greater solubility allows one to obtain solution with greater ease and work at room temperature without fear of crystallization as occurs with the sodium sulphite or hyposulphite.