Purine and pyrimidine nucleotides in ossification cartilage

Abstract

Ossifiable cartilage of new-born pigs was homogenized, set free of proteins and centrifuged. The supernatant was freedof glycogen and of acid mucopolysaccharides (chondroitinsulphates), fractionated with barium acetate and ethanol: three different precipitates were so obtained (barium salts of different nucleotides).
Nucleotides were separated by paper chromatography with two-dimensional technique. Bach nucleotide, eluted from its own chromatogram, was analyzed with Beckman spectrophotometer and spectra were recorded at different p~EL and after addition of bromine water.

Results were obtained showing that in ossifií€able cartilage of pigs adenine, guanine, cytosine and uracil nucleotides arepresent.