Modification of the technic of successive absorptions of Sols and Ponz by slow-penetrating substances

Abstract

The Sols and Ponz's method of succesive absorptions in situ as applied to the rat intestine, uses 7.5-10.00 mi as initial volume of solution. The quantity of the absorbed sugar is well determined as the difference between the initial and the residual one, when the sugars are rapidly absorbed but with pentoses or other sugars slowly absorbed, the evaluation presents some difficulties because that difference is then small.

The modification proposed by the authors consists in repla- cing the dispositive of funnels, tubes and cannulae by two poly- thene tubes of 2 at 3 mm diameter and 5 cm long which serve as inlet and outlet cannulae at the two ends of the elected intestinal loop.

A 10-20 mi syringe conected at the proximal tube, alows the repeated washing out of the loop with saline, and next, to

The Sols and Ponz's method of succesive absorptions in situ as applied to the rat intestine, uses 7.5-10.00 mi as initial volume of solution. The quantity of the absorbed sugar is well determined as the difference between the initial and the residual one, when the sugars are rapidly absorbed but with pentoses or other sugars slowly absorbed, the evaluation presents some difficulties because that difference is then small.

The modification proposed by the authors consists in repla- cing the dispositive of funnels, tubes and cannulae by two poly- thene tubes of 2 at 3 mm diameter and 5 cm long which serve as inlet and outlet cannulae at the two ends of the elected intestinal loop.

A 10-20 mi syringe conected at the proximal tube, alows the repeated washing out of the loop with saline, and next, to

The Sols and Ponz's method of succesive absorptions in situ as applied to the rat intestine, uses 7.5-10.00 mi as initial volume of solution. The quantity of the absorbed sugar is well determined as the difference between the initial and the residual one, when the sugars are rapidly absorbed but with pentoses or other sugars slowly absorbed, the evaluation presents some difficulties because that difference is then small.

The modification proposed by the authors consists in repla- cing the dispositive of funnels, tubes and cannulae by two poly- thene tubes of 2 at 3 mm diameter and 5 cm long which serve as inlet and outlet cannulae at the two ends of the elected intestinal loop.

A 10-20 mi syringe conected at the proximal tube, alows the repeated washing out of the loop with saline, and next, to

pour completely the residual saline out by passing of air. An exactly measured quantity of solution to be absorbed is put then in the syringe cylinder and is left to flow into the loop and drive out all the air. Then, the outlet tube is closed. At the end of the absorption time the residual solution is washed out with saline quantitatively for sugar determination and the loop remains ready to performe in it sucessive absorptions.

The results with D-glucose and L-arabinose 0.3 M are shown, with absorption times of 15 and 60 min. respectively. Glucose absorption is very constant in the different succesives absorp­ tions in the same animal. The first absorption of arabinose is always higher than the succesives ones, but in these last the absorption remains also constant.