Some contributions to the fractionation of blood proteins with DEAE-cellulose

Abstract

A new gradient a system is described based on phosphate buffer solutions, by means of which the pH is continuiously changed from 8.52 to 4.40 and the ionic concentration from M/100 to M/10, using an easily system of mixing.

A study has been made by means of this system of the protein fraction of the serum of different mammals (man, cow, sheep, goat, pig, horse and rat) on colums of DEAE-cellulose, and great uni­formity has been obtained in the fraction in all cases, with variations practically only of a quantitative order.

The only evident qualitative differen­ ce ies in albumin, which in some species is completely homogenous and in others appears divided into two majority frac­tions.

In every case the gamma-globulin fraction appears perfectly homogeneous in the zone of most rapid elution, and confined to a very limited number of tu­bes. These conditions are ideal for a quantitative recovery of this fraction in a biological material.