Simple elution procedure for estimation of the serum protein bands separated by disc electrophoresis in polyacrylamide gel

Abstract

Normal sera and sera from patients of several types of cancer were studied by disc electrophoresis in acrylamide gel under conditions close to those of Ornstein and Davis. After staining the proteins with aniline blue black, the main bands were section­ ed in slices for a quantitative estimation; the gel was disgregated by stirring with a rod and the protein eluted using a solution made with borate buffer (pH 9.8), acetic acid and sodium lauril sulphate. Remarkable differences were generally observed for the main fractions of cancer sera in comparison with those from normal samples, and also between the respective bands of the various groups of cancer. The three types of haptoglobins (1-1, 2-1 and 2-2) were detected. Conditions for staining glycoproteins with good result are also described.